Zcommander luke zhan p1 haat3/10/2023 Molecular analysis indicated in all cases that these phenotypes were a result of suppression of endogenous DET1 expression, due to post-transcriptional gene silencing. Unexpectedly, we only observed phenotypes characteristic of DET1 inactivation, i.e. To gain further understanding about the function of DET1 during plant development, we generated a range of overexpression constructs and introduced them into tomato. From genetic analyses it has been proposed that DET1 is a negative regulator of light signal transduction, and recent results indicate that it may control light-regulated gene expression at the level of chromatin remodelling. Summary The tomato HIGH PIGMENT-2 gene encodes an orthologue of the Arabidopsis nuclear protein DE-ETIOLATED 1 ( DET1). Manipulation of DET1 expression in tomato results in photomorphogenic phenotypes caused by post-transcriptional gene silencingĭavuluri, Ganga Rao van Tuinen, Ageeth Mustilli, Anna Chiara Manfredonia, Alessandro Newman, Robert Burgess, Diane Brummell, David A. Thus interactions between ABF genes contribute to det1 salt/osmotic stress response phenotypes. In addition, abf1 suppressed det1 rapid water loss and open stomata phenotypes. Double mutant analysis showed that abf3, abf4, and abf1 all suppress the det1 salt/osmotic stress resistant germination phenotype. We then investigated the role of ABF3, ABF4, and ABF1 in det1 phenotypes. We found that ABF3 is induced by salt in wildtype seeds, while homologues ABF4 and ABF1 are repressed, and all three genes are underexpressed in det1 seeds. Our analysis revealed that ABA regulated genes, including ABA RESPONSIVE ELEMENT BINDING FACTOR 3 (ABF3), are downregulated in det1 seedlings. We then queried publically available microarray datasets to identify genes downstream of HY5 that were differentially expressed in det1 mutants. We found that hy5 suppressed the det1 salt and mannitol resistant germination phenotype, thus, det1 stress resistant germination requires HY5. LONG HYPOCOTYL 5 (HY5) positively regulates both light and abscisic acid (ABA) signalling. While DE-ETIOLATED 1 ( DET1) is well known as a negative regulator of light development, here we describe how det1 mutants also exhibit altered responses to salt and osmotic stress, specifically salt and mannitol resistant germination. This phenotype requires HY5, ABF1, ABF3, and ABF4. Role of Arabidopsis ABF1/3/4 during det1 germination in salt and osmotic stress conditions.įernando, V C Dilukshi Al Khateeb, Wesam Belmonte, Mark F Schroeder, Dana FĪrabidopsis det1 mutants exhibit salt and osmotic stress resistant germination. GSC-13444, for Apple Macintosh computers. Both available in three versions: GSC-13374, for DEC VAX-series computers running VMS. DET and MPS written in FORTRAN-77 with some VAX FORTRAN-type extensions. Both packages limited in terms of orbital mechanics, they have sufficient capability to calculate data on eclipses and performance of arrays for circular or near-circular orbits. Results of simulation focus mainly on output of solar array and characteristics of batteries. DET and MPS perform minute-by-minute simulation of performance of power system. MPS system, Standard Power Regulator Unit (SPRU) utilized to operate array at array's peak power point. DET spacecraft power system feeds output of solar photovoltaic array and nickel cadmium batteries directly to spacecraft bus. DET/MPS - The GSFC Energy Balance Programsĭirect Energy Transfer ( DET) and MultiMission Spacecraft Modular Power System (MPS) computer programs perform mathematical modeling and simulation to aid in design and analysis of DET and MPS spacecraft power system performance in order to determine energy balance of subsystem.
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